Singleseed detection of an uncharacterised Betaflexiviridae virus in <i>Actinidia</i>


  • P.T. Austin
  • R.C. van_den_Brink
  • M.B. Horner
  • A.M. Duffy
  • P.M. Datson
  • R.M. MacDiarmid



A sensitive conventional RTPCR assay has been developed for an uncharacterised virus within the family Betaflexiviridae prompted by detection of the virus in Actinidia seedlings held in postentry quarantine that had been grown from openpollinated seed Infection is asymptomatic and presently assessed as lowtozero biosecurity risk The assay targets the RNAdependent RNA polymerase (RdRP) of the virus primer sequences (BetaF2 and BetaR2 278bp amplicon) being based on a partial RdRP sequence for the virus The assay detects the nine virus isolates found to date in a range of sample types (fresh leaf stem cambium archived RNA) The assay which includes a multiplexed internal control has been accredited by International Accreditation New Zealand for Actinidia testing To support management of the virus the assay and associated RNA extraction method have been applied to testing dried Actinidia seed directly They have been found sufficiently sensitive to detect the virus in individual seeds Initial results show efficient transmission of the virus from infected pollen to the seed




How to Cite

Austin, P., van_den_Brink, R., Horner, M., Duffy, A., Datson, P. and MacDiarmid, R. 2016. Singleseed detection of an uncharacterised Betaflexiviridae virus in &lt;i&gt;Actinidia&lt;/i&gt;. New Zealand Plant Protection. 69, (Jan. 2016), 325–325. DOI:



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