Isolation, pathogenicity and storage of New Zealand Alternaria species from Malus x domestica

Authors

  • Mary B. Horner Plant & Food Research

DOI:

https://doi.org/10.30843/nzpp.2018.71.146

Abstract

The pathogenicity of Alternaria spp. on Malus × domestica in New Zealand has not been investigated fully. Alternaria spp. were isolated from necrotic spots on leaves and fruit rots from various apple cultivars. Pathogenicity of 31 Alternaria spp. isolates was tested on excised ‘Royal Gala’, ‘Golden Delicious’ and ‘Scifresh’ leaves (wounded and unwounded) by measuring lesion diameters. Viability of Alternaria spp. grown from infected leaf material was assessed to compare various storage conditions. Alternaria spp. were consistently isolated from the central brown area of leaf lesions but could not be isolated from outside the leading edge or from the purple region surrounding the lesion. All isolates produced lesions on wounded leaves. Most isolates showed some pathogenicity on at least one cultivar, with lesions on unwounded leaves. Lesions on leaf material caused by Alternaria spp. can be stored for at least 11 weeks by freeze-drying or air-drying leaf material and storing at 0.5˚C or room temperature. Some New Zealand Alternaria spp. isolates on Malus × domestica are pathogenic on fruit and leaves. Further studies are needed to better understand the biology of this pathogen in New Zealand and determine a suitable control programme.

References

Brooks RM, Olmo HP 1972. Register of New Fruit and Nut Varieties. Berkley: University of California Press 708 p.

Cannibal PB, Bilder IV, Yli-Mattila T 2008. Species of the genus <em>Alternaria</em> on apple trees. Mikologiya i Fitopatologiya 42: 20&ndash;27.

Dingley JM 1969. Records of plants and diseases in New Zealand. New Zealand Department of Scientific and Industrial Research, Bulletin 192. 298 p.

Filajdic N, Sutton TB 1991. Identification and distribution of <em>Alternaria mali</em> on apples in North Carolina and susceptibility of different cultivars of apples to Alternaria blotch. Plant Disease 75: 1045&ndash;1048. <a href="https://doi.org/10.1094/PD-75-1045">https://doi.org/10.1094/PD-75-1045</a>

Filajdic N, Sutton TB 1995. Overwintering of <em>Alternaria mali</em>, the causal agent of Alternaria blotch of apple. Plant Disease 79: 695&ndash;698. <a href="https://doi.org/10.1094/PD-79-0695">https://doi.org/10.1094/PD-79-0695</a>

Harteveld DOC, Akinsanmi OA, Becker MF, Drenth A 2014a. Comparative fitness of <em>Alternaria</em> species causing leaf blotch and fruit spot of apple in Australia. Australasian Plant Pathology 43(5): 495&ndash;501. <a href="https://doi.org/10.1007/s13313-014-0297-4">https://doi.org/10.1007/s13313-014-0297-4</a>

Harteveld DOC, Akinsanmi OA, Drenth A 2012. Multiple <em>Alternaria</em> species groups are associated with leaf blotch and fruit spot diseases of apple in Australia. Plant Pathology 62: 289&ndash;297. <a href="https://doi.org/10.1111/j.1365-3059.2012.02637.x">https://doi.org/10.1111/j.1365-3059.2012.02637.x</a>

Harteveld DOC, Akinsanmi OA, Dullahide S, Drenth A 2014b. Sources and seasonal dynamics of <em>Alternaria</em> inoculum associated with leaf blotch and fruit spot of apples. Crop Protection 59: 35&ndash;42. <a href="https://doi.org/10.1016/j.cropro.2014.01.011">https://doi.org/10.1016/j.cropro.2014.01.011</a>

Hong SG, Maccaroni M, Figuli PJ, Pryor BM, Belisario A 2006. Polyphasic classification of <em>Alternaria</em> isolated from hazelnut and walnut fruit in Europe. Mycological Research 110: 1290&ndash;1300. <a href="https://doi.org/10.1016/j.mycres.2006.08.005">https://doi.org/10.1016/j.mycres.2006.08.005</a>

Kidd MN 1929. Apple-rot fungi in the overseas transport and marketing of New Zealand apples. New Zealand Journal of Science and Technology 11: 30&ndash;32.

Li Y, Aldwinckle HS, Sutton T, Tsuge T, Kang G, Cong P, Cheng Z 2013. Interactions of apple and the <em>Alternaria alternata</em> apple pathotype. Critical Reviews in Plant Sciences 32: 141&ndash;150. <a href="https://doi.org/10.1080/07352689.2012.722026">https://doi.org/10.1080/07352689.2012.722026</a>

Li Y, Zhang LY, Zhang Z, Cong PH and Cheng ZM 2011. A simple sequence repeat marker linked to the susceptibility of apple to alternaria blotch caused by <em>Alternaria alternata</em> apple pathotype. Journal of the American Society for Horticultural Science. 136: 109&ndash;115.

Rotondo F, Collina M, Brunelli A, Pryor BM 2012. Comparison of <em>Alternaria</em> spp. collected in Italy from apple with <em>A. mali</em> and other AM-toxin producing strains. Phytopathology 102: 1130&ndash;1142. <a href="https://doi.org/10.1094/PHYTO-04-12-0076-R">https://doi.org/10.1094/PHYTO-04-12-0076-R</a>

Sawamura K, Yukita K 2014. Alternaria blotch. In: Sutton TB, Aldwinckle HS, Agnello AM, Walgenbach JF eds. Compendium of Apple and Pear Diseases and Pests. Second edition. The American Phytopathological Society, St. Paul, Minnesota, USA. Pp. 32&ndash;33.

Serdani M, Kang JC, Andersen B, Crous PW 2002. Characterisation of <em>Alternaria</em> species-groups associated with core rot of apples in South Africa. Mycological Research 106: 561&ndash;569. <a href="https://doi.org/10.1017/S0953756202005993">https://doi.org/10.1017/S0953756202005993</a>

Simmons EG 1999. <em>Alternaria</em> themes and variations (236-243) - host-specific toxin producers. Mycotaxon 70: 325&ndash;369.

Simmons EG 2007. <em>Alternaria</em>: An Identification Manual. Utrecht: CBS Fungal Biodiversity Centre.

Suzuki H, Kume Y, Matsui I 1987. Effect of early defoliation on growth and fruit quality of apple trees. Bulletin of the Akita Fruit-Tree Experiment Station: 1&ndash;13.

Toome-Heller M, Baskarathevan J, Burnip G, Alexander B 2018. First report of apple leaf blotch caused by <em>Alternaria arborescens</em> complex in New Zealand. New Zealand Journal of Crop and Horticultural Science. <a href="https://doi.org/">https://doi.org/</a> 10.1080/01140671.2018.1427117

Volz RK, White AG, Brookfield PL, Weskett R, Legg S, Koorey R, Tustin DS, Hughes J,White M 2004. Breeding and development of the new apple cultivar &lsquo;JazzTM&rsquo;. Acta Horticulturae 663: 891&ndash;894. <a href="https://doi.org/10.17660/ActaHortic.2004.663.162">https://doi.org/10.17660/ActaHortic.2004.663.162</a>

Published

2018-07-29

How to Cite

Horner, M. B. (2018). Isolation, pathogenicity and storage of New Zealand Alternaria species from Malus x domestica. New Zealand Plant Protection, 71, 166-173. https://doi.org/10.30843/nzpp.2018.71.146

Issue

Section

Vol 71 Apple Pathogens 2018