Comparison of the behaviour of a transformed hygromycin resistant strain of <i>Trichoderma atroviride</i> (M1057hygR) with the wildtype strain (M1057)

Authors

  • K.L. McLean
  • S.L. Dodd
  • B.E. Sleight
  • R.A. Hill
  • A. Stewart

DOI:

https://doi.org/10.30843/nzpp.2004.57.6892

Abstract

The biocontrol isolate Trichoderma atroviride M1057 and a transformed hygromycin resistant biotype (M1057hygR) were compared using biological control rhizosphere competence and antibiosis studies to determine whether the transformed biotype performed in a similar manner to the wildtype strain In an onion growth chamber trial using soil naturally infested with the onion white rot pathogen Sclerotium cepivorum there was no significant difference (P>005) in the level of disease control given by the two T atroviride strains Similarly populations of T atroviride M1057 and M1057hygR were equivalent (P>005) in the rhizosphere of onion seedlings There was no significant difference (P>005) between the mycelial growth rates of S cepivorum when grown on agar amended with culture filtrate of T atroviride M1057 and M1057hygR Thus T atroviride M1057hygR has similar biological attributes to the wildtype isolate and can be used in future field studies looking at the population ecology of the biological control agent

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Published

2004-08-01

How to Cite

McLean, K.L., S.L. Dodd, B.E. Sleight, R.A. Hill, and A. Stewart. “Comparison of the Behaviour of a Transformed Hygromycin Resistant Strain of &lt;i&gt;Trichoderma atroviride&lt;/i&Gt; (M1057hygR) With the Wildtype Strain (M1057)”. New Zealand Plant Protection 57 (August 1, 2004): 72–76. Accessed October 24, 2021. https://journal.nzpps.org/index.php/nzpp/article/view/6892.

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