Time and temperature combinations that are lethal to <i>Pseudomonas syringae</i> pv <i>actinidiae</i>


  • I.P.S. Pushparajah
  • K.R. Everett




The thermal death point was determined in vitro for three isolates of Pseudomonas syringae pv actinidiae (Psa) A bacterial suspension was treated by placing in an Eppendorf tube then floating in a water bath at seven temperatures from 263 to 60C At each temperature an aliquot was removed after 5 15 35 75 155 315 and 635 min placed onto Kings medium B (KMB) in Petri plates and spread with a sterilised bent glass rod Petri plates were placed at 25C for 24 h and colonies counted There were three isolate replicates each in an Eppendorf tube per timetemperature combination There was no difference in response between isolate replicates but time and temperature differences were significant (P<00001) Any timetemperature combination greater than 45C for 5 min killed Psa Modelling the thermal response suggested that 425C for longer than 150 min was the lowest timetemperature combination that could be used to kill the bacterial cells




How to Cite

Pushparajah, I. and Everett, K. 2011. Time and temperature combinations that are lethal to &lt;i&gt;Pseudomonas syringae&lt;/i&gt; pv &lt;i&gt;actinidiae&lt;/i&gt;. New Zealand Plant Protection. 64, (Jan. 2011), 291–291. DOI:https://doi.org/10.30843/nzpp.2011.64.6002.



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