A simple PCRRFLP method to distinguish between species and strains of <i>Microctonus</i> parasitoids found in New Zealand


  • C.J. Vink




Two strains of the hymenopteran parasitoid Microctonus aethiopoides have been released in New Zealand for the biological control of Sitona weevil species One attacks Sitona discoideus a pest of lucerne and the other attacks Sitona lepidus a pest of clover Two other Microctonus species also attack weevils in pasture; M hyperodae was released for the biological control of Listronotus bonariensis and the native M zealandicus attacks Irenimus spp These Microctonus species can attack nontarget weevil hosts and the identification of the larvae of the different Microctonus species and the separation of adults of M aethiopoides strains can only be achieved by molecular methods This paper describes a simple polymerase chain reaction and restriction fragment length polymorphism (PCRRFLP) method for distinguishing between the two M aethiopoides strains M hyperodae and M zealandicus This PCRRFLP method requires minimal molecular equipment and is cheaper and/or faster than other molecular methods




How to Cite

Vink, C.J. “A Simple PCRRFLP Method to Distinguish Between Species and Strains of &lt;i&gt;Microctonus&lt;/i&gt; Parasitoids Found in New Zealand”. New Zealand Plant Protection 65 (January 8, 2012): 186–191. Accessed February 25, 2024. https://journal.nzpps.org/index.php/nzpp/article/view/5392.