A simple PCRRFLP method to distinguish between species and strains of <i>Microctonus</i> parasitoids found in New Zealand
AbstractTwo strains of the hymenopteran parasitoid Microctonus aethiopoides have been released in New Zealand for the biological control of Sitona weevil species One attacks Sitona discoideus a pest of lucerne and the other attacks Sitona lepidus a pest of clover Two other Microctonus species also attack weevils in pasture; M hyperodae was released for the biological control of Listronotus bonariensis and the native M zealandicus attacks Irenimus spp These Microctonus species can attack nontarget weevil hosts and the identification of the larvae of the different Microctonus species and the separation of adults of M aethiopoides strains can only be achieved by molecular methods This paper describes a simple polymerase chain reaction and restriction fragment length polymorphism (PCRRFLP) method for distinguishing between the two M aethiopoides strains M hyperodae and M zealandicus This PCRRFLP method requires minimal molecular equipment and is cheaper and/or faster than other molecular methods
How to Cite
Vink, C.J. “A Simple PCRRFLP Method to Distinguish Between Species and Strains of <i>Microctonus</i> Parasitoids Found in New Zealand”. New Zealand Plant Protection 65 (January 8, 2012): 186–191. Accessed February 25, 2024. https://journal.nzpps.org/index.php/nzpp/article/view/5392.