Detection of <i>Phaeomoniella chlamydospora</i> in soil using speciesspecific PCR

Authors

  • S.A. Whiteman
  • M.V. Jaspers
  • A. Stewart
  • H.J. Ridgway

DOI:

https://doi.org/10.30843/nzpp.2002.55.3943

Abstract

Phaeomoniella chlamydospora is a fungal pathogen of woody grapevine tissue that causes Petri disease As one potential source of inoculum is infested soil a SDS/phenol/chloroform DNA extraction method and PCR assay utilising speciesspecific primers were evaluated for the ability to detect P chlamydospora in grapevine nursery soil Using a nested PCR approach the assay was able to detect 102 conidia/ml when a spore suspension was added to sterilised soil samples and 50 fg when P chlamydospora genomic DNA was added directly to the reaction In this process preamplification of a 600 bp region of the ribosomal DNA was followed by amplification with primers Pch 1 and Pch 2 to produce a 360 bp speciesspecific product This highly sensitive diagnostic tool will be used in future studies to determine if pathogen propagules are present in soils collected from New Zealand grapevine nurseries

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Published

2002-08-01

How to Cite

Whiteman, S., Jaspers, M., Stewart, A., & Ridgway, H. (2002). Detection of <i>Phaeomoniella chlamydospora</i> in soil using speciesspecific PCR. New Zealand Plant Protection, 55, 139-145. https://doi.org/10.30843/nzpp.2002.55.3943

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