Detection of <i>Phaeomoniella chlamydospora</i> in soil using speciesspecific PCR
AbstractPhaeomoniella chlamydospora is a fungal pathogen of woody grapevine tissue that causes Petri disease As one potential source of inoculum is infested soil a SDS/phenol/chloroform DNA extraction method and PCR assay utilising speciesspecific primers were evaluated for the ability to detect P chlamydospora in grapevine nursery soil Using a nested PCR approach the assay was able to detect 102 conidia/ml when a spore suspension was added to sterilised soil samples and 50 fg when P chlamydospora genomic DNA was added directly to the reaction In this process preamplification of a 600 bp region of the ribosomal DNA was followed by amplification with primers Pch 1 and Pch 2 to produce a 360 bp speciesspecific product This highly sensitive diagnostic tool will be used in future studies to determine if pathogen propagules are present in soils collected from New Zealand grapevine nurseries
How to Cite
Whiteman, S.A., M.V. Jaspers, A. Stewart, and H.J. Ridgway. “Detection of <i>Phaeomoniella chlamydospora</i> In Soil Using Speciesspecific PCR”. New Zealand Plant Protection 55 (August 1, 2002): 139–145. Accessed July 26, 2021. https://journal.nzpps.org/index.php/nzpp/article/view/3943.