Elicitor induction of defence genes and reduction of bacterial canker in kiwifruit

  • K.V. Wurms The New Zealand Institute for Plant & Food Research Limited, Ruakura, Private Bag 3230, Waikato Mail Centre, Hamilton 3240, New Zealand
  • E. Gould Zespri International Limited, P.O. Box 4043, Mount Maunganui South, New Zealand
  • A. Ah Chee The New Zealand Institute for Plant & Food Research Limited, Ruakura, Private Bag 3230, Waikato Mail Centre, Hamilton 3240, New Zealand
  • J. Taylor The New Zealand Institute for Plant & Food Research Limited, Ruakura, Private Bag 3230, Waikato Mail Centre, Hamilton 3240, New Zealand
  • B. Curran The New Zealand Institute for Plant & Food Research Limited, Mount Albert Research Centre, Private Bag 92169, Mount Albert, Auckland 1142, New Zealand; School of Biological Sciences, Faculty of Science, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand
  • T. Reglinski The New Zealand Institute for Plant & Food Research Limited, Ruakura, Private Bag 3230, Waikato Mail Centre, Hamilton 3240, New Zealand
Keywords: Pseudomonas syringae pv actinidiae, Psa, bacterial canker, kiwifruit, acibenzolar- S-methyl, elicitor, defence genes, transcript expression, qPCR

Abstract

Pseudomonas syringae pv. actinidiae (Psa), which causes bacterial canker, is the most serious global pathogen of kiwifruit. Like most bacterial pathogens, control options are limited, but elicitors can reduce disease significantly, particularly those that induce the salicylic acid (SA) pathway. Acibenzolar-S-methyl (ASM), a SA analogue, is one of the most effective elicitors for Psa control. In this study, real-time PCR (qPCR) was used to measure the expression of 18 putative defence genes in Actinidia chinensis var. chinensis ‘Hort16A’ in response to Psa and ASM. Application of ASM led to up-regulation of RPM1 interacting protein 4 (RIN4), phenylalanine ammonia lyase (PAL), a hypersensitivity-induced response protein (HIRP), and β-1,3-glucosidase. Expression of PAL and HIRP was further enhanced when elicitor application and Psa-inoculation were combined. Elevated gene expression was correlated with decreased disease expression, and supports the hypothesis that elicitor-treated plants are primed to react more rapidly and/or strongly to pathogens.

Published
2017-07-26
How to Cite
Wurms, K., Gould, E., Ah Chee, A., Taylor, J., Curran, B., & Reglinski, T. (2017). Elicitor induction of defence genes and reduction of bacterial canker in kiwifruit. New Zealand Plant Protection, 70, 272-284. https://doi.org/https://doi.org/10.30843/nzpp.2017.70.61
Section
Vol 70 Kiwifruit & Grape Pathology 2017