Development of phenotyping assays for resistance screening of blueberry plants against <i>Neofusicoccum</i> spp.

  • R.E. Campbell The New Zealand Institute for Plant & Food Research Ltd, Nelson, Old Mill Road, RD3, Motueka, 7198, New Zealand
  • H. Walters The New Zealand Institute for Plant & Food Research Ltd, Nelson, Old Mill Road, RD3, Motueka, 7198, New Zealand
  • K.M.S. Tennakoon Faculty of Agriculture and Life Sciences, Lincoln University, PO Box 85084, Lincoln, New Zealand
  • E.E. Jones Faculty of Agriculture and Life Sciences, Lincoln University, PO Box 85084, Lincoln, New Zealand
  • M. Walter The New Zealand Institute for Plant & Food Research Ltd, Nelson, Old Mill Road, RD3, Motueka, 7198, New Zealand

Abstract

Botryosphaeria disease is a common problem on a large range of hosts, including blueberry, causing dieback and cankers. Methods for phenotyping assays were developed in this project to facilitate blueberry resistance breeding. Detached shoots were used to test effects of wound size (needle, scalpel), inoculum type (mycelium, conidia), stem hardness (soft, semi-soft) and incubation temperatures on lesion development. Experiments were carried out on various blueberry cultivars with four Neofusicoccum strains, two each of N. parvum and N. ribis. Lesions of similar lengths developed on both scalpel and needle wounded shoots indicating no wound size effects. Mycelium plugs produced lesions faster than conidial suspension. Similarly, lesions on soft wood progressed faster than those on semi-soft cuttings. Incubation temperature had a significant effect on lesion development, with 26°C showing faster disease development than 19 or 22°C. This information will inform assays for blueberry plant breeding for improved disease resistance.

Published
2017-07-24
How to Cite
CAMPBELL, R.E. et al. Development of phenotyping assays for resistance screening of blueberry plants against Neofusicoccum spp.. New Zealand Plant Protection, [S.l.], v. 70, p. 215-223, july 2017. ISSN 1179-352X. Available at: <http://journal.nzpps.org/index.php/nzpp/article/view/53>. Date accessed: 25 nov. 2017.
Section
Rice & Blueberry Pathology

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.