A simple method for conidial production and establishing latent infections of apples by <i>Phlyctema vagabunda</i> (syn: <i>Neofabraea alba</i>)

  • K.R. Everett The New Zealand Institute for Plant & Food Research Limited, Mt Albert Research Centre, Private Bag 92169, Mt Albert, Auckland 1142, New Zealand
  • I.P.S. Pushparajah The New Zealand Institute for Plant & Food Research Limited, Mt Albert Research Centre, Private Bag 92169, Mt Albert, Auckland 1142, New Zealand
  • B.M. Fisher Plant & Food Research, Havelock North Research Centre, Private Bag 1401, Havelock North, New Zealand
  • P.N. Wood Plant & Food Research, Havelock North Research Centre, Private Bag 1401, Havelock North, New Zealand
Keywords: Latency, quiescent infections, bull’s eye rot, ‘Scilate’/Envy™, rapid conidial production

Abstract

A method was developed to inoculate and establish infection of detached apple fruit with the bull’s eye rot pathogen, Phlyctema vagabunda (syn: Neofabraea alba), without wounding. Mycelial cultures of P. vagabunda did not produce conidia on commonly used potato dextrose agar, or several other tested media. Growth on corn meal agar resulted in the highest conidial yield, and maximum production was achieved after 5 days. These conidia were placed on water agar, and apple fruit were placed on these spores for at least 5 days for establishment of infections. Reliable infection of 66–100% of apples required 14 days of contact. The ability to rapidly produce copious quantities of conidia and inoculate without wounding facilitates a number of other postharvest control and epidemiology studies.

Published
2017-08-08
How to Cite
Everett, K., Pushparajah, I., Fisher, B., & Wood, P. (2017). A simple method for conidial production and establishing latent infections of apples by <i>Phlyctema vagabunda</i> (syn: <i>Neofabraea alba</i&gt;). New Zealand Plant Protection, 70, 106-111. https://doi.org/https://doi.org/10.30843/nzpp.2017.70.35
Section
Vol 70 Apple Pathology 2017